Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Antioxidant activity and antibacterial evaluation of new thiazolin-4-one derivatives as potential tryptophanyl-tRNA synthetase inhibitors

doi: 10.1080/14756366.2019.1596086

Figure Lengend Snippet: The top binding affinity of compounds to E. coli TrpRS and S. aureus TrpRS, the computed inhibition constant and the mean binding affinities of compounds’ conformations in the 2 Å cluster.

Article Snippet: There are many validated primary structures of TrpRS isolated from various strains of S. aureus deposited in UniProt: P67593 – (strain N315), P67592 – strain Mu50/ATCC 700699, Q5HH88 – strain COL, Q6GI89 – strain MRSA252, Q6GAT0 – strain MSSA476, P67594 – strain MW2, T1Y8L7 from S. aureus subsp. aureus CN1, A0A0E0VMI8 from S. aureus subsp. aureus 71193, A0A0E1AGS6 from S. aureus subsp. aureus Z172, Q2FZQ7 – strain NCTC 8325, A0A0E1VIN1 from S. aureus subsp. aureus USA300_TCH959, A0A0E1X8M2 from S. aureus subsp. aureus MN8, A0A0H3KFE9 – strain Newman, A0A0H2XGX6 – strain USA300, A0A2H5AMI2 - strain 046.

Techniques: Binding Assay, Inhibition

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Antioxidant activity and antibacterial evaluation of new thiazolin-4-one derivatives as potential tryptophanyl-tRNA synthetase inhibitors

doi: 10.1080/14756366.2019.1596086

Figure Lengend Snippet: Polar contacts made by the top binding conformation of each compound to the catalytic site of TrpRS.

Article Snippet: There are many validated primary structures of TrpRS isolated from various strains of S. aureus deposited in UniProt: P67593 – (strain N315), P67592 – strain Mu50/ATCC 700699, Q5HH88 – strain COL, Q6GI89 – strain MRSA252, Q6GAT0 – strain MSSA476, P67594 – strain MW2, T1Y8L7 from S. aureus subsp. aureus CN1, A0A0E0VMI8 from S. aureus subsp. aureus 71193, A0A0E1AGS6 from S. aureus subsp. aureus Z172, Q2FZQ7 – strain NCTC 8325, A0A0E1VIN1 from S. aureus subsp. aureus USA300_TCH959, A0A0E1X8M2 from S. aureus subsp. aureus MN8, A0A0H3KFE9 – strain Newman, A0A0H2XGX6 – strain USA300, A0A2H5AMI2 - strain 046.

Techniques: Binding Assay, Residue

Journal: Frontiers in Microbiology

Article Title: Insights in the Antimicrobial Potential of the Natural Nisin Variant Nisin H

doi: 10.3389/fmicb.2020.573614

Figure Lengend Snippet: Determination of purity, yield and cleavage efficiency of the pre-nisin variants. (A) Purity of the purified nisin A and the variants nisin H and nisin H F 1 I (Marker: Precision Plus Protein Dual Xtra standards Bio-Rad). (B) Yields after purification of nisin A and their corresponding variants via cation-exchange chromatography. (C) Quantification of the cleavage efficiency of NisP. (D) Chromatogram of nisin H. (E) Chromatogram of the nisin H F 1 I variant. The pre-nisin variants before NisP cleavage were shown by blue dashed lines and after NisP cleavage by black lines. Error bars represent the standard deviation of at least three biological replicates.

Article Snippet: All bacteria used for minimum inhibitory concentration (MIC) determination of nisin variants [ Bacillus subtilis 168; S. aureus : MSSA strain ATCC 29213, MRSA/VISA strain ATCC 700699; E. faecium : ATCC 35667, ATCC 700221 (vancomycin resistant); E. faecalis : ATCC 29212, ATCC 51299 (vancomycin resistant)] were cultivated in Mueller-Hinton broth (MHB) at 37°C and shaking at 150 rpm.

Techniques: Purification, Marker, Chromatography, Variant Assay, Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Insights in the Antimicrobial Potential of the Natural Nisin Variant Nisin H

doi: 10.3389/fmicb.2020.573614

Figure Lengend Snippet: Nisin mediated pore formation, visualized with the SYTOX green assay. The NZ9000Cm strain incubated with the SYTOX dye. After a stable baseline (∼200 s), one of the nisin variants (100 nM) was added (indicated with an arrow). The fluorescence signal was measured using a fluorolog (Horiba III). The rapid increase in fluorescence indicates pore formation. The black line represents the addition of nisin A, the red line nisin H, and the blue line nisin H F 1 I. As a control we added buffer shown as green line.

Article Snippet: All bacteria used for minimum inhibitory concentration (MIC) determination of nisin variants [ Bacillus subtilis 168; S. aureus : MSSA strain ATCC 29213, MRSA/VISA strain ATCC 700699; E. faecium : ATCC 35667, ATCC 700221 (vancomycin resistant); E. faecalis : ATCC 29212, ATCC 51299 (vancomycin resistant)] were cultivated in Mueller-Hinton broth (MHB) at 37°C and shaking at 150 rpm.

Techniques: Incubation, Fluorescence, Control

Journal: Frontiers in Microbiology

Article Title: Insights in the Antimicrobial Potential of the Natural Nisin Variant Nisin H

doi: 10.3389/fmicb.2020.573614

Figure Lengend Snippet: MIC values for nisin A, nisin H, and nisin H F 1 I against different pathogenic strains.

Article Snippet: All bacteria used for minimum inhibitory concentration (MIC) determination of nisin variants [ Bacillus subtilis 168; S. aureus : MSSA strain ATCC 29213, MRSA/VISA strain ATCC 700699; E. faecium : ATCC 35667, ATCC 700221 (vancomycin resistant); E. faecalis : ATCC 29212, ATCC 51299 (vancomycin resistant)] were cultivated in Mueller-Hinton broth (MHB) at 37°C and shaking at 150 rpm.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Current State of Knowledge Regarding WHO High Priority Pathogens—Resistance Mechanisms and Proposed Solutions through Candidates Such as Essential Oils: A Systematic Review

doi: 10.3390/ijms24119727

Figure Lengend Snippet: Studies assessing the antimicrobial activity of essential oils against methicillin resistant, vancomycin-intermediate and -resistant S. aureus (a non-exhaustive list).

Article Snippet: Demirci F et al., 2018 , MRSA ATCC 700699 S. aureus ATCC BAA-1026 S. epidermidis ATCC 14990 S. pyogenes ATCC 19615 S. pneumoniae ATCC 10015 P. aeruginosa ATCC 10145 H. influenzae ATCC 49247 M. catarrhalis ATCC 23245 , Thymus sipyleus Boiss. , Agar diffusion Broth microdilution Vapour diffusion , The best antibacterial effect for thyme scented lemon was noticed against S. aureus , S. pyogenes and M. catarrhalis (MIC = 12–13 μg/mL). MRSA MIC = 310 μg/mL and P. aeruginosa MIC = 1250 μg/mL (lowest inhibitory effect). The anti-inflammatory effect was 12.1 ± 1.8% in 100 μg/mL, both effects being required for efficient inhalations in the treatment of rhinosinusitis. , [ ] .

Techniques: Activity Assay, Diffusion-based Assay, Inhibition, Cytotoxicity Assay, Electron Microscopy, In Vitro, Dilution Assay, Preserving, Quantitative Proteomics, Nucleic Acid Electrophoresis, Membrane, Confocal Laser Scanning Microscopy, Concentration Assay, Titration, Bacteria, Microscopy, Transmission Assay, Microdilution Assay, Produced, Modification, Clinical Proteomics, Blocking Assay, Staining, Cell Culture, Fourier Transform Infrared Spectroscopy, Spectroscopy, Reverse Transcription, Real-time Polymerase Chain Reaction, Crystal Violet Assay, Expressing, Flow Cytometry, In Vivo, Liposomes, Time-Kill Assay, Formulation, MTT Assay, Incubation, Thin Layer Chromatography, Bioassay, Antibiofilm Assay, Resazurin Assay, Biofilm Production Assay, Control, Infection, Cream, Antioxidant Activity Assay, Permeability, Virus, Extraction, Isolation